His Tag ELISA Detection Kit, a 1.5 hour competition ELISA, is developed for rapid and high throughput detection of His-tagged proteins.

產品型號：L00436

更新時間：2025-07-16

廠商性質：代理商

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產品介紹

Note

L00436 interferes with IgG in mouse/rat serum and is not suitable for detecting mouse/rat serum samples.

產品概述

Product Description

GenScript His Tag ELISA Detection Kit, a 1.5 hour competition ELISA, is developed for rapid and high throughput detection of His-tagged proteins. There are several potential usages:

●Quickly identify the presence of His-tagged proteins in samples.

●Optimize protein expression by monitoring the His-tagged proteins level.

●High throughput screening of stable cell lines expressing His-tagged proteins.

產品簡介

Detection Range ：1 ng/ml~729 ng/ml

Sensitivity： 1 ng/ml His-tagged proteins

Test Samples： N-terminal/C-terminal/internal His-tagged proteins4 x His/5 x His/6 x His-tagged proteins

Mammalian, yeast and bacteria cell lysates or cell supernatant

Conveniency ：Provide all reagents required for test

Complete test within 1.5 hours

Reagents compatibility： Tolerable with various reagents at certain concentration

Storage ：The unopened kit is stable for at least 12 months if stored at 2-8 °C and the opened kit may be stable for up to 1 month at 2-8 °C. Do not freeze the kit.

Kit Contents：

Component	Quantity	Part. No
His Tag Plate	1 plate (8 wells × 12 strips)	436-80
Anti-His Monoclonal Antibody	6 ml	436-20
Antibody Tracer	12 ml	436-30
His Tag Standards (0,1,3,9,27,81,243,729 ng/ml)	1 ml per each standard	436-11,12,13,14,15,16,17,18
Assay Diluent	60 ml	436-60
20 × Wash Solution	40 ml	B-A1-70
TMB Solution	12 ml	B-A1-40
Stop Solution	6 ml	B-A1-50
His Tag Standard Stock (10μg/ml)	500 μl	436-10
Plate Sealer	2	N/A